Laboratory Protocols

General Procedures

• General Comments
• Administrative rules/procedures
• Safety and waste disposal
• Biohazard bag
• Sharps containers
• Recycling bins
• Regular trash
• Daily procedures
• Weekly procedures
• Sorted by day
• Monthly procedures
• Miscellaneous (BUT IMPORTANT) procedures
• Wash glassware
• Deionized water stock
• Autoclave
• Physiology solution preparation
• pH Meter calibration
• Electrode glass preparation
• Sylgard
• Others
• Sutter Pipette Puller

Molecular Biology Procedures

• Restriction digest
• Isolating DNA from the restriction digest reaction mixture
• Miniprep
• DNA gel
• Standard PCR
• RT-PCR
• Thermocycling
• PCR debugging
• Purification of PCR products
• PCR directly from colonies
• Ligation
• RNA synthesis
• RNA gel
• DNA sequencing
• Transformation of EColi with plasmid DNA
• Maxiprep
• Oligo stocks
• A typical PCR mediated mutagenesis
• Electroporation competent EColi preparation
• Yeast growth assay
• Yeast two-hybrid assay
• Plasmid DNA from yeast
• Molecular Biology Reagents/Solutions
• Transfection of mammalian cells

Oocyte Procedures

• Frog Surgery
• RNA injection
• Oocyte maintenance

Non-oocyte cell culture protocols

• Changing the cell medium

Dissociation of rat/mouse neurons

• Dissociation protocol

Two-electrode voltage clamp

Cut-open clamp

Patch-clamp

Analysis

Job list for the lab undergraduate help

Salines for Recording from BK channels

Salines for oocytes

Salines for Recording Ca²⁺ currents

Instructions for using Ca²⁺ electrode

Injection of oocytes

Removing vitelline membrane from oocytes